Induction of Diploid Gynogenesis in Wels Catfish (Silurus glanis) Using UV-Irradiated Grass Carp (Ctenopharyngodon idella) Sperm

This article describes the first successful induction of meiotic gynogenesis in Wels catfish (Silurus glanis) using heterologous sperm of grass carp (Ctenopharyngodon idella) with DNA inactivated by means of UV-irradiation at 4800 J/m(2). The cold shock at 6 degrees C (duration of 20 min) started in the nineth minute after fertilization was found as efficient in retaining the second polar body and producing meiotic diploids. Successful induction of meiotic gynogenesis was confirmed by the microsatellite DNA analysis, which revealed only maternal inheritance in gynogenetic offspring. Ploidy of gynogenetic offspring was verified by the application of active nucleoli counting analysis. J. Exp. Zool 373A:24-27, 2010. (C) 2009 Wiley-Liss, Inc.