期刊
JOURNAL OF EXPERIMENTAL MEDICINE
卷 211, 期 6, 页码 1137-1151出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20132267
关键词
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资金
- National Institutes of Health (NIH) [R01 GM106173, AI038339, R21 AI099825, R01 GM082940]
- Howard Hughes Medical Institute
- University of Chicago Digestive Diseases Research Core Center [P30 DK42086]
- Defense Advanced Research Projects Agency [D12AP00023]
- Cancer Research Institute
Induction of Bcl6 (B cell lymphoma 6) is essential for T follicular helper (Tfh) cell differentiation of antigen-stimulated CD4(+) T cells. Intriguingly, we found that Bcl6 was also highly and transiently expressed during the CD4(+) CD8(+) (double positive [DP]) stage of T cell development, in association with the E3 ligase cullin 3 (Cul3), a novel binding partner of Bcl6 which ubiquitinates histone proteins. DP stage- specific deletion of the E3 ligase Cul3, or of Bcl6, induced the derepression of the Bcl6 target genes Batf (basic leucine zipper transcription factor, ATF-like) and Bcl6, in part through epigenetic modifications of CD4(+) single-positive thymocytes. Although they maintained an apparently normal phenotype after emigration, they expressed increased amounts of Batf and Bcl6 at basal state and produced explosive and prolonged Tfh responses upon subsequent antigen encounter. Ablation of Cul3 in mature CD4(+) splenocytes also resulted in dramatically exaggerated Tfh responses. Thus, although previous studies have emphasized the essential role of Bcl6 in inducing Tfh responses, our findings reveal that Bcl6-Cul3 complexes also provide essential negative feedback regulation during both thymocyte development and T cell activation to restrain excessive Tfh responses.
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