期刊
ONCOLOGY REPORTS
卷 34, 期 1, 页码 368-374出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2015.3996
关键词
hepatocellular carcinoma; miR-193b; Mcl-1; cisplatin; apoptosis; caspase-3
类别
资金
- National Natural Science Foundation of China [81172575]
- Hunan Provincial Natural Science Committee
- Hengyang City Government Unification Foundation of China [12JJ9033]
- Hunan Provincial Natural Science Foundation of China [13JJ3079]
Mounting evidence suggests that microRNAs (miRNAs) play important roles in the development of cancer by targeting expression of tumor-related genes. In the present study, downregulation of miR-193b was observed in hepatocellular carcinoma (HCC) tissues and HCC cell lines by quantitative RT-PCR analyses, suggesting that miR-193b is a tumor-suppressor in HCC. More importantly, miR-193b significantly enhanced the cytotoxicity of cisplatin in HepG2 cells by targeting Mc1-1. Knockdown of the Mc1-1 gene by specific siRNA exhibited a function similar to miR-193b on sensitizing HepG2 cells to cisplatin-inducing cytotoxicity. Furthermore, the miR-193b-induced sensitization of HepG2 cells to cisplatin cytotoxicity was abolished by the transfection of Mc-1 expression plasmid that lacked the 3'-untranslated region (3'-UTR). In addition, activation of caspase-3 was needed for sensitization by miR-193b to cisplatin-mediated cell death. Thus, the present study revealed the downregulation of miR-193b in HCC cells and illustrated a synergistic effect on cisplatin-induced apoptosis by targeting Mcl-1.
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