期刊
JOURNAL OF EXPERIMENTAL MEDICINE
卷 210, 期 13, 页码 2887-2902出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20131026
关键词
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资金
- HICCC
- CLL Global Research Foundation (USA)
- National Cancer Institute/National Institutes of Health (NCI/NIH) through fellowships of the Fondazione Cariplo (Italy) [R21-CA175461]
- American-Italian Cancer Foundation (USA)
- German Research Council (DFG)
- Cancer Biology Training Program fellowship (NCI/NIH) [5T32-CA009503-26]
The transcription factor interferon regulatory factor-4 (IRF4) is expressed in B cells at most developmental stages. In antigen-activated B cells, IRF4 controls germinal center formation, class-switch recombination, and the generation of plasma cells. Here we describe a novel function for IRF4 in the homeostasis of mature B cells. Inducible deletion of irf4 specifically in B cells in vivo led to the aberrant accumulation of irf4-deleted follicular B cells in the marginal zone (MZ) area. IRF4-deficient B cells showed elevated protein expression and activation of NOTCH2, a transmembrane receptor and transcriptional regulator known to be required for MZ B cell development. Administration of a NOTCH2-inhibitory antibody abolished nuclear translocation of NOTCH2 in B cells within 12 h and caused a rapid and progressive disintegration of the MZ that was virtually complete 48 h after injection. The disappearance of the MZ was accompanied by a transient increase of MZ-like B cells in the blood rather than increased B cell apoptosis, demonstrating that continued NOTCH2 activation is critical for the retention of B cells in the MZ. Our results suggest that IRF4 controls the positioning of mature B cells in the lymphoid microenvironments by regulating NOTCH2 expression. These findings may have implications for the understanding of B cell malignancies with dysregulated IRF4 and NOTCH2 activity.
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