期刊
JOURNAL OF EXPERIMENTAL MEDICINE
卷 210, 期 4, 页码 699-714出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20122609
关键词
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资金
- Interdisziplinaren Zentrum fur Klinische Forschung (Ulm)
- LFS Defekte bei Aufbau und Erhalt von Immunfunktionen
- Deutsche Forschungsgemeinschaft [FE 578/3-1]
Expression of the pre-T cell receptor alpha (pT alpha) gene has been exploited in previous studies as a molecular marker to identify tiny cell populations in bone marrow (BM) and blood that were suggested to contain physiologically relevant thymus settling progenitors (TSPs). But to what extent these cells genuinely contribute to thymopoiesis has remained obscure. We have generated a novel pT alpha(iCre) knockin mouse line and performed lineage-tracing experiments to precisely quantitate the contribution of pT alpha-expressing progenitors to distinct differentiation pathways and to the genealogy of mature hematopoietic cells under physiological in vivo conditions. Using these mice in combination with fluorescent reporter strains, we observe highly consistent labeling patterns that identify pT alpha expression as a faithful molecular marker of T lineage commitment. Specifically, the fate of pT alpha-expressing progenitors was found to include all alpha beta and most gamma delta T cells but, in contrast to previous assumptions, to exclude B, NK, and thymic dendritic cells. Although we could detect small numbers of T cell progenitors with a history of pT alpha expression in BM and blood, our data clearly exclude these populations as physiologically important precursors of thymopoiesis and indicate that they instead belong to a pathway of T cell maturation previously defined as extrathymic.
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