期刊
JOURNAL OF EXPERIMENTAL MEDICINE
卷 206, 期 5, 页码 1037-1046出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20082179
关键词
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资金
- Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan [17790766, 19591303, 19591249, 195229, 18390311, 19659281]
- National Institutes of Health [AI063331, AR051790]
- Grants-in-Aid for Scientific Research [19591249, 19659281, 18390311, 21390324, 21390310, 19591303, 17790766] Funding Source: KAKEN
Urticarial rash observed in cryopyrin-associated periodic syndrome (CAPS) caused by nucleotide-binding oligomerization domain-leucine-rich repeats containing pyrin domain 3 (NLRP3) mutations is effectively suppressed by anti-interleukin (IL)-1 treatment, suggesting a pathophysiological role of IL-1. in the skin. However, the cellular mechanisms regulating IL-1. production in the skin of CAPS patients remain unclear. We identified mast cells (MCs) as the main cell population responsible for IL-1. production in the skin of CAPS patients. Unlike normal MCs that required stimulation with proinflammatory stimuli for IL-1. production, resident MCs from CAPS patients constitutively produced IL-1 beta. Primary MCs expressed inflammasome components and secreted IL-1. via NLRP3 and apoptosis-associated speck-like protein containing a caspase recruitment domain when stimulated with microbial stimuli known to activate caspase-1. Furthermore, MCs expressing disease-associated but not wild-type NLRP3 secreted IL-1. and induced neutrophil migration and vascular leakage, the histological hallmarks of urticarial rash, when transplanted into mouse skin. Our findings implicate MCs as IL-1. producers in the skin and mediators of histamine-independent urticaria through the NLRP3 inflammasome.
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