期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 65, 期 8, 页码 2147-2160出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/eru084
关键词
COI1; jasmonate; primary metabolism; starch; tobacco
资金
- Fundamental Research Funds for the Central Universities of China [XDJK2012C084]
- China National Tobacco Corporation [110201101009 (JY-03)]
- 22nd Century LLC [GF12288]
- State Education Ministry (SRF for ROCS)
- Southwest University [SWU110053]
NtCOI1, a tobacco orthologue of the Arabidopsis jasmonate co-receptor COI1, was shown to regulate primary carbohydrate metabolism in both reproductive and vegetative organs of Nicotiana tabacum L. cv. TN90.Jasmonate (JA) plays an important role in regulating plant male fertility and secondary metabolism, but its role in regulating primary metabolism remains unclear. The F-box protein CORONATINE INSENSITIVE 1 (COI1) is a critical component of the JA receptor, and mediates JA-signalling by targeting JASMONATE ZIM-domain (JAZ) proteins for proteasomal degradation in response to JA perception. Here, we found that RNA interference-mediated knockdown of NtCOI1 in tobacco (Nicotiana tabacum L. cv. TN90) recapitulated many previously observed phenotypes in coi1 mutants, including male sterility, JA insensitivity, and loss of floral anthocyanin production. It also affected starch metabolism in the pollen, anther wall, and floral nectary, leading to pollen abortion and loss of floral nectar. Transcript levels of genes encoding starch metabolism enzymes were significantly altered in the pollen, anther wall, and floral nectary of NtCOI1-silenced tobacco. Changes in leaf primary metabolism were also observed in the NtCOI1-silenced tobacco. The expression of NtMYB305, an orthologue of MYB305 previously identified as a flavonoid metabolic regulator in Antirrhinum majus flowers and as a floral-nectar regulator mediating starch synthesis in ornamental tobacco, was extremely downregulated in NtCOI1-silenced tobacco. These findings suggest that NtCOI1 functions upstream of NtMYB305 and plays a fundamental role in coordinating plant primary carbohydrate metabolism and correlative physiological processes.
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