期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 65, 期 15, 页码 4149-4157出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/eru189
关键词
Rice; root hair; type I cell wall; type II cell wall; xyloglucan; xylosyltransferase
资金
- Key Basic Research Special Foundation of China [2011CB100303]
- National Science Foundation of China [31172024, 31201675]
- Ministry of Science and Technology of China [2010DFA31080, 2014ZX08009328-002]
- Australian Research Council Centre of Excellence [CEO561495, CE140100008]
Root hairs are important for nutrient uptake, anchorage, and plant-microbe interactions. From a population of rice (Oryza sativa) mutagenized by ethyl methanesulfonate (EMS), a short root hair2 (srh2) mutant was identified. In hydroponic culture, srh2 seedlings were significantly reduced in root hair length. Bubble-like extrusions and irregular epidermal cells were observed at the tips of srh2 root hairs when grown under acidic conditions, suggesting the possible reduction of the tensile strength of the cell wall in this mutant. Map-based cloning identified a mutation in the gene encoding xyloglucan (XyG) 6-xylosyltransferase (OsXXT1). OsXXT1 displays more than 70% amino acid sequence identity with the previously characterized Arabidopsis thaliana XYG XYLOSYL TRANSFERASE 1 (AtXXT1) and XYG XYLOSYL TRANSFERASE 2 (AtXXT2), which catalyse the transfer of xylose onto beta-1,4-glucan chains. Furthermore, expression of the full-length coding sequence of OsXXT1 could complement the root hair defect, and slow growth and XyG synthesis in the Arabidopsis xxt1 xxt2 double mutant. Transgenic plants expressing the beta-glucuronidase (GUS) reporter under the control of the OsXXT1 promoter displayed GUS expression in multiple tissues, most prominently in root epidermal cells. These results demonstrate the importance of OsXXT1 in maintaining cell wall structure and tensile strength in rice, a typical grass species that contains relatively low XyG content in cell walls.
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