期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 65, 期 15, 页码 4119-4131出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/eru184
关键词
Abscisic acid; drought stress; in vivo; neuronal nitric oxide synthase; nitric oxide; physiological; PYL; transcriptomic
资金
- National Natural Science Foundation of China [31370302, 31200194]
- Knowledge Innovative Key Program of the Chinese Academy of Sciences [54Y154761O01076, Y329631O0263]
- Chinese Academy of Sciences
- US National Institutes of Health [R01GM059138]
- Youth Innovation Promotion Association of the Chinese Academy of Sciences [Y429371O04]
- Outstanding Young Talent Program of Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture [Y352811O03, Y452331O03]
Nitric oxide (NO) is involved in plant responses to many environmental stresses. Transgenic Arabidopsis lines that constitutively express rat neuronal NO synthase (nNOS) were described recently. In this study, it is reported that the nNOS transgenic Arabidopsis plants displayed high levels of osmolytes and increased antioxidant enzyme activities. Transcriptomic analysis identified 601 or 510 genes that were differentially expressed as a consequence of drought stress or nNOS transformation, respectively. Pathway and gene ontology (GO) term enrichment analyses revealed that genes involved in photosynthesis, redox, stress, and phytohormone and secondary metabolism were greatly affected by the nNOS transgene. Several CBF genes and members of zinc finger gene families, which are known to regulate transcription in the stress response, were changed by the nNOS transgene. Genes regulated by both the nNOS transgene and abscisic acid (ABA) treatments were compared and identified, including those for two ABA receptors (AtPYL4 and AtPYL5). Moreover, overexpression of AtPYL4 and AtPYL5 enhanced drought resistance, antioxidant enzyme activity, and osmolyte levels. These observations increase our understanding of the role of NO in drought stress response in Arabidopsis.
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