期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 65, 期 15, 页码 4451-4464出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/eru219
关键词
Abscisic acid (ABA); drought resistance; tomato ABA receptor; tomato clade A PP2C
资金
- Ministerio de Ciencia e Innovacion
- Fondo Europeo de Desarrollo Regional
- Consejo Superior de Investigaciones Cientificas [BIO2011-23446, BFU2011-25384, COST1106]
- Juan de la Cierva
- Direct For Biological Sciences [1258175] Funding Source: National Science Foundation
- Division Of Integrative Organismal Systems [1258175] Funding Source: National Science Foundation
Abscisic acid (ABA) plays a crucial role in the plant's response to both biotic and abiotic stress. Sustainable production of food faces several key challenges, particularly the generation of new varieties with improved water use efficiency and drought tolerance. Different studies have shown the potential applications of Arabidopsis PYR/PYL/RCAR ABA receptors to enhance plant drought resistance. Consequently the functional characterization of orthologous genes in crops holds promise for agriculture. The full set of tomato (Solanum lycopersicum) PYR/PYL/RCAR ABA receptors have been identified here. From the 15 putative tomato ABA receptors, 14 of them could be grouped in three subfamilies that correlated well with corresponding Arabidopsis subfamilies. High levels of expression of PYR/PYL/RCAR genes was found in tomato root, and some genes showed predominant expression in leaf and fruit tissues. Functional characterization of tomato receptors was performed through interaction assays with Arabidopsis and tomato clade A protein phosphatase type 2Cs (PP2Cs) as well as phosphatase inhibition studies. Tomato receptors were able to inhibit the activity of clade A PP2Cs differentially in an ABA-dependent manner, and at least three receptors were sensitive to the ABA agonist quinabactin, which inhibited tomato seed germination. Indeed, the chemical activation of ABA signalling induced by quinabactin was able to activate stress-responsive genes. Both dimeric and monomeric tomato receptors were functional in Arabidopsis plant cells, but only overexpression of monomeric-type receptors conferred enhanced drought resistance. In summary, gene expression analyses, and chemical and transgenic approaches revealed distinct properties of tomato PYR/PYL/RCAR ABA receptors that might have biotechnological implications.
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