期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 66, 期 5, 页码 1291-1302出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/eru479
关键词
Biomechanics; cell wall; fasciclin-like arabinogalactan protein; Populus; transgenic plant; xylem
资金
- National Natural Science Foundation of China [31000288, 31171169, 31100212, 31371228, 31370670]
- National Basic Research Program of China [2010CB126600]
- National Mega Project of GMO Crops [2013ZX08001003-007, 2013ZX08004002-006, 2014ZX0800942B]
- National Natural Science Foundation of Jiangsu Province of China [BK2012306]
- Chinese Academy of Sciences [XDA08030108]
Fasciclin-like arabinogalactan proteins (FLAs) play important roles in the growth and development of roots, stems, and seeds in Arabidopsis. However, their biological functions in woody plants are largely unknown. In this work, we investigated the possible function of PtFLA6 in poplar. Quantitative real-time PCR, PtFLA6-yellow fluorescent protein (YFP) fusion protein subcellular localization, Western blotting, and immunohistochemical analyses demonstrated that the PtFLA6 gene was expressed specifically in the xylem of mature stem, and PtFLA6 protein was distributed ubiquitous in plant cells and accumulated predominantly in stem xylem fibres. Antisense expression of PtFLA6 in the aspen hybrid clone Poplar davidianaxPoplar bolleana reduced the transcripts of PtFLA6 and its homologous genes. Transgenic plants that showed a significant reduction in the transcripts of PtFLAs accumulated fewer PtFLA6 and arabinogalactan proteins than did the non-transgenic plants, leading to reduced stem flexural strength and stiffness. Further studies revealed that the altered stem biomechanics of transgenic plants could be attributed to the decreased cellulose and lignin composition in the xylem. In addition expression of some xylem-specific genes involved in cell wall biosynthesis was downregulated in these transgenic plants. All these results suggest that engineering the expression of PtFLA6 and its homologues could modulate stem mechanical properties by affecting cell wall composition in trees.
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