期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 64, 期 12, 页码 3843-3854出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/ert216
关键词
ADP-glucose pyrophosphorylase; Arabidopsis thaliana; chloroplast; NTRC; ROS; starch; thioredoxins
资金
- National Science Foundation
- Academy of Finland [127819, 130192]
- Finnish Graduate School in Plant Biology
- Turku University Foundation
- Carl and Thecla Lamberg Foundation
- Academy of Finland (AKA) [127819, 130192, 127819, 130192] Funding Source: Academy of Finland (AKA)
Plastid-localized NADPH-dependent thioredoxin reductase C (NTRC) is a unique NTR enzyme containing both reductase and thioredoxin domains in a single polypeptide. Arabidopsis thaliana NTRC knockout lines (ntrc) show retarded growth, especially under short-day (SD) photoperiods. This study identified chloroplast processes that accounted for growth reduction in SD-acclimated ntrc. The strongest reduction in ntrc growth occurred under photoperiods with nights longer than 14h, whereas knockout of the NTRC gene did not alter the circadian-clock-controlled growth of Arabidopsis. Lack of NTRC modulated chloroplast reactive oxygen species (ROS) metabolism, but oxidative stress was not the primary cause of retarded growth of SD-acclimated ntrc. Scarcity of starch accumulation made ntrc leaves particularly vulnerable to photoperiods with long nights. Direct interaction of NTRC and ADP-glucose pyrophosphorylase, a key enzyme in starch synthesis, was confirmed by yeast two-hybrid analysis. The ntrc line was not able to maximize starch synthesis during the light period, which was particularly detrimental under SD conditions. Acclimation of Arabidopsis to SD conditions also involved an inductive rise of ROS production in illuminated chloroplasts that was not counterbalanced by the activation of plastidial anti-oxidative systems. It is proposed that knockout of NTRC challenges redox regulation of starch synthesis, resulting in stunted growth of the mutant lines acclimated to the SD photoperiod.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据