期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 62, 期 10, 页码 3563-3573出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/err046
关键词
Cadmium; gene regulation; heavy metal tolerance; microarray; microRNA; rice; stress response
资金
- Zhejiang Provincial Natural Science Foundation of China [Z3100327]
- National Nature Science Foundation of China [31071348]
- State Key Laboratory of Rice Biology, China National Rice Research Institute [090102]
- Zhejiang Innovation Program for Graduates
MicroRNAs (miRNAs) are a class of small non-coding RNAs that negatively regulate specific target mRNAs at the post-transcriptional level. Plant miRNAs have been implicated in developmental processes and adaptations to environmental stresses. Cadmium (Cd) is a non-essential heavy metal that is highly toxic to plants. To investigate the responsive functions of miRNAs under Cd stress, miRNA expression in Cd-stressed rice (Oryza sativa) was profiled using a microarray assay. A total of 19 Cd-responsive miRNAs were identified, of which six were further validated experimentally. Target genes were also predicted for these Cd-responsive miRNAs, which encoded transcription factors, and proteins associated with metabolic processes or stress responses. In addition, the mRNA levels of several targets were negatively correlated with the corresponding miRNAs under Cd stress. Promoter analysis showed that metal stress-responsive cis-elements tended to occur more frequently in the promoter regions of Cd-responsive miRNAs. These findings suggested that miRNAs played an important role in Cd tolerance in rice, and highlighted a novel molecular mechanism of heavy metal tolerance in plants.
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