期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 62, 期 14, 页码 5063-5078出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/err211
关键词
Arabidopsis; cell wall; homogalacturonan; methyltransferase; pectin; plant suspension cultured cells; S-adenosyl-L-methionine
资金
- Research Grants Council of Hong Kong [CUHK488707, CUHK465708, CUHK466309, CUHK466610]
- CUHK Schemes B/C
- Department of Energy [DE-FG09-93ER-20097]
Pectins are complex polysaccharides that are essential components of the plant cell wall. In this study, a novel putative Arabidopsis S-adenosyl-L-methionine (SAM)-dependent methyltransferase, termed QUASIMODO 3 (QUA3, At4g00740), has been characterized and it was demonstrated that it is a Golgi-localized, type II integral membrane protein that functions in methylesterification of the pectin homogalacturonan (HG). Although transgenic Arabidopsis seedlings with overexpression, or knock-down, of QUA3 do not show altered phenotypes or changes in pectin methylation, this enzyme is highly expressed and abundant in Arabidopsis suspension-cultured cells. In contrast, in cells subjected to QUA3 RNA interference (RNAi) knock-down there is less pectin methylation as well as altered composition and assembly of cell wall polysaccharides. Taken together, these observations point to a Golgi-localized QUA3 playing an essential role in controlling pectin methylation and cell wall biosynthesis in Arabidopsis suspension cell cultures.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据