Dynamics of leaf hydraulic conductance with water status: quantification and analysis of species differences under steady state

Leaf hydraulic conductance (K-leaf) is a major determinant of photosynthetic rate in well-watered and drought-stressed plants. Previous work assessed the decline of K-leaf with decreasing leaf water potential (Psi(leaf)), most typically using rehydration kinetics methods, and found that species varied in the shape of their vulnerability curve, and that hydraulic vulnerability correlated with other leaf functional traits and with drought sensitivity. These findings were tested and extended, using a new steady-state evaporative flux method under high irradiance, and the function for the vulnerability curve of each species was determined individually using maximum likelihood for 10 species varying strongly in drought tolerance. Additionally, the ability of excised leaves to recover in K-leaf with rehydration was assessed, and a new theoretical framework was developed to estimate how rehydration of measured leaves may affect estimation of hydraulic parameters. As hypothesized, species differed in their vulnerability function. Drought-tolerant species showed shallow linear declines and more negative Psi(leaf) at 80% loss of K-leaf (P-80), whereas drought-sensitive species showed steeper, non-linear declines, and less negative P-80. Across species, the maximum K-leaf was independent of hydraulic vulnerability. Recovery of K-leaf after 1 h rehydration of leaves dehydrated below their turgor loss point occurred only for four of 10 species. Across species without recovery, a more negative P-80 correlated with the ability to maintain K-leaf through both dehydration and rehydration. These findings indicate that resistance to K-leaf decline is important not only in maintaining open stomata during the onset of drought, but also in enabling sustained function during drought recovery.