期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 61, 期 13, 页码 3599-3613出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erq173
关键词
Cotton fibre; EMSA; HD-Zip IV transcription factor; L1 box; R2R3-MYB transcription factor; SAD domain; START domain; yeast two-hybrid
资金
- China `973' Program [2004CB117303-3, 2007CB108805]
- China 863'' Program [2010AA100503]
- China Transgenic Research Program [2008ZX08002-001]
- Shanghai Leading Academic Discipline Project [B209]
Transcription factors play key roles in plant development through their interaction with cis-elements and/or other transcription factors. A HD-Zip IV family transcription factor, Gossypium barbadense Meristem Layer 1 (GbML1) has been identified and characterized here. GbML1 specifically bound to the L1 box and the promoters of GbML1 and GbRDL1. GbML1 physically interacted with a key regulator of cotton fibre development, GbMYB25. Truncated and point mutation assays indicated the START-SAD domain was required for the binding to the C terminal domain (CTD) of GbMYB25. GbML1 overexpression in Arabidopsis increased the number of trichomes on stems and leaves and increased the accumulation of anthocyanin in leaves. Taken together, the L1 box binding protein, GbML1 was identified as the first partner for GbMYB25 and the role of START domain was discovered to be a protein binding domain in plants. Our findings will help the improvement of cotton fibre production and the understanding of the key role of HD-Zip family and MYB family in plants.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据