期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 60, 期 4, 页码 1309-1318出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erp005
关键词
HT-B; Petunia inflata; pistil; RNAi; self-incompatibility
资金
- Ministry of Education, Science, Sports and Culture of Japan [B, 20380003]
Gametophytic self-incompatibility (GSI) in Solanaceae, Rosaceae, and Plantaginaceae is controlled by a multiallelic S-locus. The specificities of pistil and pollen are controlled by separate S-locus genes, S-RNase and SLF/SFB, respectively. Although the S-specificity is determined by the S-locus genes, factors located outside the S-locus are also required for expression of GSI. HT-B is one of the pistil non-S-factors identified in Nicotiana and Solanum, and encodes a small asparagine/aspartate-rich extracellular protein with unknown biochemical function. Here, HT-B was cloned from Petunia and characterized. The structural features and expression pattern of Petunia HT-B were very similar to those of Nicotiana and Solanum. Unlike other solanaceous species, expression of HT-B was also observed in self-compatible Petunia species. RNA interference (RNAi)-mediated suppression of Petunia HT-B resulted in partial breakdown of GSI. Quantitative analysis of the HT-B mRNA accumulation in the transgenics showed that a 100-fold reduction is not sufficient and a > 1000-fold reduction is required to achieve partial breakdown of GSI.
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