期刊
ONCOGENE
卷 35, 期 20, 页码 2615-2623出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/onc.2015.317
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资金
- Universite Paris Descartes
- Ligue nationale contre le cancer, Canceropole Ile-de-France
- Fondation ARC Association pour la Recherche sur le Cancer
- Fondation pour la Recherche Medicale
- Institut National du Cancer [INCA_6508]
- Ligue nationale contre le cancer comite de Paris
- Region Pays-de-la-Loire
- Nantes Metropole
Glioblastoma are malignant highly vascularized brain tumours, which feature large oedema resulting from tumour-promoted vascular leakage. The pro-permeability factor Semaphorin3A (Sema3A) produced within glioblastoma has been linked to the loss of endothelial barrier integrity. Here, we report that extracellular vesicles (EVs) released by patient-derived glioblastoma cells disrupt the endothelial barrier. EVs expressed Sema3A at their surface, which accounted for in vitro elevation of brain endothelial permeability and in vivo vascular permeability, in both skin and brain vasculature. Blocking Sema3A or its receptor Neuropilin1 (NRP1) hampered EV-mediated permeability. In vivo models using ectopically and orthotopically xenografted mice revealed that Sema3A-containing EVs were efficiently detected in the blood stream. In keeping with this idea, sera from glioblastoma multiforme (GBM) patients also contain high levels of Sema3A carried in the EV fraction that enhanced vascular permeability, in a Sema3A/NRP1-dependent manner. Our results suggest that EV-delivered Sema3A orchestrates loss of barrier integrity in glioblastoma and may be of interest for prognostic purposes.
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