期刊
JOURNAL OF EXPERIMENTAL BIOLOGY
卷 216, 期 19, 页码 3724-3732出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jeb.089243
关键词
microRNA; microarrays; RNAi; social behavior
类别
资金
- Research Council of Norway [180504, 191699, 213976]
- Pew Charitable Trust
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil [481000/2009-7]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), Brazil [11/03171-5]
- CNPq [161917/2011-9]
- FAPESP [07/07594-2]
- Arizona State University
- Smithsonian Tropical Research Institute
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [07/07594-2] Funding Source: FAPESP
In honey bees, vitellogenin (Vg) is hypothesized to be a major factor affecting hormone signaling, food-related behavior, immunity, stress resistance and lifespan. MicroRNAs, which play important roles in post-transcriptional gene regulation, likewise affect many biological processes. The actions of microRNAs and Vg are known to intersect in the context of reproduction; however, the role of these associations on social behavior is unknown. The phenotypic effects of Vg knockdown are best established and studied in the forager stage of workers. Thus, we exploited the well-established RNA interference (RNAi) protocol for Vg knockdown to investigate its downstream effects on microRNA population in honey bee foragers' brain and fat body tissue. To identify microRNAs that are differentially expressed between tissues in control and knockdown foragers, we used mu Paraflo microfluidic oligonucleotide microRNA microarrays. Our results showed that 76 and 74 microRNAs were expressed in the brain of control and knockdown foragers whereas 66 and 69 microRNAs were expressed in the fat body of control and knockdown foragers, respectively. Target prediction identified potential seed matches for a differentially expressed subset of microRNAs affected by Vg knockdown. These candidate genes are involved in a broad range of biological processes including insulin signaling, juvenile hormone (JH) and ecdysteroid signaling previously shown to affect foraging behavior. Thus, here we demonstrate a causal link between the Vg knockdown forager phenotype and variation in the abundance of microRNAs in different tissues, with possible consequences for the regulation of foraging behavior.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据