期刊
JOURNAL OF EXPERIMENTAL BIOLOGY
卷 213, 期 1, 页码 118-127出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jeb.037077
关键词
calcitonin-like diuretic hormone (CLDH); cardiac ganglion (CG); cardioactive peptide; diuretic hormone 31 (DH31); expressed sequence tag (EST); heart; neurohormone; neuromodulator; neuropeptide; reverse transcriptase polymerase chain reaction (RT-PCR); transcriptomics; Homarus americanus
类别
资金
- National Center for Research Resources [P20 RR-016463]
- MDIBL
- Nevada Agriculture Experiment Station [NEV000337]
- NATIONAL CENTER FOR RESEARCH RESOURCES [P20RR016463] Funding Source: NIH RePORTER
In insects, a family of peptides with sequence homology to the vertebrate calcitonins has been implicated in the control of diuresis, a process that includes mixing of the hemolymph. Here, we show that a member of the insect calcitonin-like diuretic hormone (CLDH) family is present in the American lobster, Homarus americanus, serving, at least in part, as a powerful modulator of cardiac output. Specifically, during an ongoing EST project, a transcript encoding a putative H. americanus CLDH precursor was identified; a full-length cDNA was subsequently cloned. In silico analyses of the deduced prepro-hormone predicted the mature structure of the encoded CLDH to be GLDLGLGRGFSGSQAAKHLMGLAAANFAGGPamide (Homam-CLDH), which is identical to a known Tribolium castaneum peptide. RT-PCR tissue profiling suggests that Homam-CLDH is broadly distributed within the lobster nervous system, including the cardiac ganglion (CG), which controls the movement of the neurogenic heart. RTPCR analysis conducted on pacemaker neuron-and motor neuron-specific cDNAs suggests that the motor neurons are the source of the CLDH message in the CG. Perfusion of Homam-CLDH through the isolated lobster heart produced dose-dependent increases in both contraction frequency and amplitude and a dose-dependent decrease in contraction duration, with threshold concentrations for all parameters in the range 10(-11) to 10(-10). mol. l(-1) or less, among the lowest for any peptide on this system. This report is the first documentation of a decapod CLDH, the first demonstration of CLDH bioactivity outside the Insecta, and the first detection of an intrinsic neuropeptide transcript in the crustacean CG.
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