Long-term changes in the chlorophyll fluorescence of bleached and recovering corals from Hawaii

Chlorophyll fluorescence has been used to predict and monitor coral bleaching over short timescales ( hours to days), but long-term changes during recovery remain largely unknown. To evaluate changes in fluorescence during long-term bleaching and recovery, Porites compressa and Montipora capitata corals were experimentally bleached in tanks at 30 C for 1 month, while control fragments were maintained at 27 degrees C. A pulse amplitude modulated fluorometer measured the quantum yield of photosystem II fluorescence (F(v)/F(m)) of the zooxanthellae each week during bleaching, and after 0, 1.5, 4 and 8 months recovery. M. capitata appeared bleached 6days sooner than P. compressa, yet their fluorescence patterns during bleaching did not significantly differ. Changes in minimum (F(o)), maximum (F(m)) and variable (F(v)) fluorescence throughout bleaching and recovery indicated periods of initial photoprotection followed by photodamage in both species, with P. compressa requiring less time for photosystem II (PS II) repair than M. capitata. F(v)/F(m) fully recovered 6.5 months earlier in P. compressa than M. capitata, suggesting that the zooxanthellae of P. compressa were more resilient to bleaching stress.