期刊
JOURNAL OF EUKARYOTIC MICROBIOLOGY
卷 61, 期 6, 页码 655-671出版社
WILEY
DOI: 10.1111/jeu.12176
关键词
Apicoplast; drug target; genetic manipulation; knockout; malaria; metabolism; phenotype
类别
资金
- Melbourne Research Scholarship from University of Melbourne
- School of Botany
- National Health and Medical Research Council
- Australian Research Council
Malaria plagues one out of every 30 humans and contributes to almost a million deaths, and the problem could worsen. Our current therapeutic options are compromised by emerging resistance by the parasite to our front line drugs. It is thus imperative to better understand the basic biology of the parasite and develop novel drugs to stem this disease. The most facile approach to analyse a gene's function is to remove it from the genome or inhibit its activity. Although genetic manipulation of the human malaria parasite Plasmodium falciparum is a relatively standard procedure, there is no optimal method to perturb genes essential to the intraerythrocytic development cyclethe part of the life cycle that produces the clinical manifestation of malaria. This is a severe impediment to progress because the phenotype we wish to study is exactly the one that is so elusive. In the absence of any utilitarian way to conditionally delete essential genes, we are prevented from investigating the parasite's most vulnerable points. This review aims to focus on the development of tools identifying essential genes of P. falciparum and our ability to elicit phenotypic mutation.
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