期刊
JOURNAL OF EUKARYOTIC MICROBIOLOGY
卷 59, 期 2, 页码 185-187出版社
WILEY
DOI: 10.1111/j.1550-7408.2011.00602.x
关键词
Ciliophora; environmental sequencing; Illumina Solexa; 454 Pyrosequencing
类别
资金
- Alexander von Humboldt Foundation
- Deutsche Forschungsgemeinschaft [STO414/3-1]
The hyper-variable V4 and V9 regions of the small subunit (SSU) rDNA have been targeted for assessing environmental diversity of microbial eukaryotes using next generation sequencing technologies. Here, we explore how the genetic distances among these short fragments compare with the distances obtained from near full-length SSU-rDNA sequences by comparing all pairwise estimates, as well as within and among species of ciliates. Results show that pairwise distances from V4 more closely match the near full-length SSU-rDNA and are more comparable with previous studies based on much longer SSU-rDNA fragments, then pairwise distances from V9. Thus, studies that use the V4 will estimate similar values of phylotype richness and community structure as would have been estimated using the full-length SSU-rDNA.
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