期刊
JOURNAL OF EUKARYOTIC MICROBIOLOGY
卷 57, 期 5, 页码 400-404出版社
WILEY
DOI: 10.1111/j.1550-7408.2010.00499.x
关键词
Diplmonad fish parasite; Hexamitidae; hydrogenase; mass spectrometry; metronidazole; Spironucleus vortens
类别
资金
- William E. Morgan Scholarship
- Natural Environment Research Council, UK [NER/J/S/2002/00706]
- Natural Environment Research Council [NER/J/S/2002/00706] Funding Source: researchfish
The diplomonad fish parasite Spironucleus vortens causes major problems in aquaculture of ornamental fish, resulting in severe economic losses in the fish farming industry. The strain of S. vortens studied here was isolated from an angelfish and grown in Keister's modified TY-I-S33 medium. A membrane-inlet mass spectrometer was employed to monitor, in a closed system, O-2, CO2, and H-2. When introduced into air-saturated buffer, S. vortens rapidly consumed O-2 at the average rate of 62 +/- 4 nmol/min/107 cells and CO2 was produced at 75 +/- 11 nmol/min/107 cells. Hydrogen production began under microaerophilic conditions ([O-2]=33.+/- 15 mu M) at a rate of 77 +/- 7 nmol/min/107 cells. Hydrogen production was inhibited by 62% immediately after adding 150 mu M KCN to the reaction vessel, and by 50% at 0.24 mu M CO, suggesting that an Fe-only hydrogenase is responsible for H-2 production. Metronidazole (1 mM) inhibited H-2 production by 50%, while CO2 production was not affected. This suggests that metronidazole may be reduced by an enzyme of the H-2 pathway, thus competing for electrons with H+.
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