期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 150, 期 3, 页码 1114-1118出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2013.10.043
关键词
Poria cocos; Bioactive fractions; Diuretic activity; Urinary excretion; Electrolyte excretion
资金
- Program for the Changjiang Scholars and Innovative Research Team in University [IRT1174]
- National Natural Science Foundation of China [81001622]
- China Postdoctoral Science Foundation [2012M521831]
- Key Program for the International S&T Cooperation Projects of Shaanxi Province [2013KW31-01]
- project As a Major New Drug to Create a Major National Science and Technology Special [2011ZX09401-308-034, 2014ZX09304-307-02]
- Natural Science Foundation of Shaanxi Provincial Education Department [2013JK0811]
- Administration of Traditional Chinese Medicine of Shaanxi [13-ZY006]
Ethnopharmacological relevance: Poria cocos Wolf (Polyporaceae) is a well-known medicinal fungus, the epidermis (Fu-Ling-Pi in Chinese) of the sclerotia is used as a diuretic for treating oedema and promoting the diuretic process. In this paper we report on the diuretic activity in rats of petroleum ether, ethyl acetate, n-butanol and the remaining fractions of the ethanol extract from the epidermis of Poria cocos. Materials and methods: Petroleum ether, ethyl acetate, n-butanol and the remaining fractions of the ethanol extract of Fu-Ling-Pi were orally administered to rats. The urinary excretion rate and the pH and electrolyte excretion were measured in the urine of saline-loaded rats. Results: In this study, all the tested fractions of Fu-Ling-Pi increased the urinary excretion rate. The three doses of the ethyl acetate fraction all produced remarkable urinary output in 6 h, and all produced a remarkable increase in Na+ excretion and Cl- excretion. The Na+/K+ value in the experimental group was significantly enhanced compared with that of the control group, but the three doses of the ethyl acetate fraction had no effect on the K+ excretion. The 25-mg/kg and 50-mg/kg doses of the n-butanol fraction showed notable urinary output and produced a remarkable increase of Na+ excretion and Cl- excretion, but the two doses did not produce a remarkable effect on the Na+/K+ value. The petroleum ether and remaining fractions did not show remarkable diuretic activities compared with the control group. Conclusions: This study confirmed that the ethyl acetate and n-butanol fractions present a remarkable diuretic effect, showing that they are the diuretic bioactive fractions of Fu-Ling-Pi. This finding appears to indicate at least two mechanisms for the observed diuretic activity, and the K+-saving diuretic effect may be related to the triterpenoid components of intermediate polarity contained in this fungus, particularly the lanostanes tetracyclic triterpenoids. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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