TG accumulation inhibitory effects of Jinqi formula by AMPK signaling pathway

Ethnophannacological relevance: Jinqi formula is a traditional Chinese anti-diabetic formula containing three ingredients (Coptidis rhizoma, Astragali rhadix and Lonicerae japonicae Flos). Materials and methods: The active fractions of Jinqi formula were purified and HPLC analyses were used for quality control. The anti-adipogenic effects of Jinqi formula were analyzed in vitro using 313-L1 cells and in vivo with KK-A(Y) mice. RT-PCR and Western blot were used to confirm genes and proteins of interest, respectively. Results: In vitro study showed that jinqi formula suppressed the accumulation of triglyceride (TG) and free fatty acids (FFA) in mature 3T3-L1 cells by increasing the expression and tyrosine phosphorylation of 5'-AMP-activated protein kinase (AMPK), as well as decreasing be expression of Acetyl CoA Carboxylase (ACC), Fatty Acid Synthase (FAS) and Hormone Sensitive Lipase (HSL). In vivo study demonstrated that Jinqi formula reduced body weight without changing food intake in KK-A(Y) mice, and decreased the levels of serum glucose, TG, FFA. In addition, consistent with the in vitro study results, Jinqi formula increased the expression and tyrosine phosphorylation of AMPK in the liver and muscular tissues of the KK-A(Y) mice. Furthermore, Jingqi formula suppressed the expression of ACC and HSL and upregulated the expression of IRS-1 in the liver. Whereas in the skeletal muscles, Jingqi formula decreased the expression of ACC and increased the expression of GLUT-4 and IRS-2. Conclusions: jingqi formula inhibits TG accumulation at least in part via the stimulation of AMPK activity in a multi-target manner. Crown Copyright (c) 2012 Published by Elsevier Ireland Ltd. All rights reserved.