期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 136, 期 1, 页码 188-196出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2011.04.038
关键词
Olive leaf extract; Diabetic neuropathic pain; Thermal hyperalgesia; Apoptosis; NGF-treated PC12 cells; Rats
资金
- Razi Herbal Medicines Research Center
- Kerman Neuroscience Research Center [KNRC/89-27]
Aim of study: Since the leaves of olive have been recommended in the literature as a remedy for the treatment of diabetes and they also contain antioxidant agents, we decided to investigate the possible effects of olive leaf extract (OLE) on in vitro and in vivo models of diabetic pain neuropathy. Materials and methods: The high glucose-induced cell damage in naive and NGF-treated Pheochromocytoma (PC12) cells and streptozotocin-induced diabetic rats were used. Tail-flick test was used to access nociceptive threshold. Cell viability was determined by MTT assay. Biochemical markers of neural apoptosis were evaluated using immunoblotting. Results: We found that elevation of glucose (4 times of normal) sequentially increases functional cell damage and caspase-3 activation in NGF-treated PC12 cells. Incubation of cells with OLE (200, 400 and 600 mu g/ml) decreased cell damage. Furthermore, the diabetic rats developed neuropathic pain which was evident from decreased tail-flick latency (thermal hyperalgesia). Activated caspase 3 and Bax/Bcl2 ratio were significantly increased in spinal cord of diabetic animals. OLE treatment (300 and 500 mg/kg per day) ameliorated hyperalgesia, inhibited caspase 3 activation and decreased Bax/Bcl2 ratio. Furthermore, OLE exhibited potent DPPH free radical scavenging capacity. Conclusion: The results suggest that olive leaf extract inhibits high glucose-induced neural damage and, suppresses diabetes-induced thermal hyperalgesia. The mechanisms of these effects may be due, at least: in part, to reduce neuronal apoptosis and suggest therapeutic potential of olive leaf extract in attenuation of diabetic neuropathic pain. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据