Effect of an extract of Andrographis paniculata leaves on inflammatory and allergic mediators in vitro

Aim of study: Andrographis paniculata has been known to possess widespread traditional application in the treatment of allergy and inflammatory diseases. In the current study, we sought to examine the effects of an extract of Andrographis paniculata leaves on inhibition of lipopolysaccharide (LPS) induced [nitric oxide (NO), prostaglandin E-2 (PGE(2)), interleukin-lbeta (IL-1 beta), and interleukin-6 (IL-6)] and calcimycin (A23187) induced lleukotriene B-4 (LTB4), thromboxane B-2 (TXB2) and histamine] mediators in diverse cell based models. Materials and methods: Effect of an extract of Andrographis paniculata leaves (AP) was studied on inhibition of LPS induced NO, PGE(2), IL-1 beta and IL-6 in J774A.1 murine macrophages; A23187 induced LTB4 and TXB2 in HL-60 promyelocytic leukemic cells and histamine in RBL-2H3 rat basophilic leukemia cells. Results and conclusion: AP illustrated significant alleviation of pro-inflammatory, inflammatory, and allergic mediators. However, no inhibition was observed against histamine release. This outcome has been summed up to deduce that AP is fairly potent in attenuating the inflammation by inhibiting pro-inflammatory (NO, IL-1 beta and IL-6), inflammatory (PGE(2) and TXB2) and allergic (LTB4) mediators. (C) 2010 Elsevier Ireland Ltd. All rights reserved.