期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 127, 期 3, 页码 625-630出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2009.12.012
关键词
Neuroinflammation; Rat astrocytoma cell line (C6); Lipo-polysaccharide; Guggulipid; Nimesulide
资金
- Senior Research Fellowship of Indian Council of Medical Research, New Delhi, India
Aim of the study: The present study was designed to investigate effect of guggulipid, a drug developed by CDRI and nimesulide on LPS stimulated neuroinflammatory changes in rat astrocytoma cell line (C6). Materials and methods: Rat astrocytoma cells (C6) were stimulated with LPS (10 mu g/ml) alone and in combinations with different concentrations of guggulipid or nimesulide for 24 h of incubation. Nitrite release in culture supernatant, ROS in cells, expressions of COX-2, GFAP and TNF-alpha in cell lysate were estimated. Results: LPS (10 mu g/ml) stimulated C6 cells to release nitrite, ROS generation, up regulated COX-2 and GFAP expressions at protein level and TNF-alpha at mRNA level. Both guggulipid and nimesulide significantly attenuated nitrite release, ROS generation and also down regulated expressions of COX-2, GFAP and TNF-alpha. Guggulipid and nimesulide per se did not have any significant effect on C6 cells. Conclusion: Results demonstrate the anti-inflammatory effect of guggulipid comparable to nimesulide which suggest potential use of guggulipid in neuroinflammation associated conditions in CNS disorders. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据