Antimicrobial activity of the methanolic extract and compounds from Morus mesozygia stem bark

Aim of the study: This study was aimed at investigating the antimicrobial activity of the methanolic extract (MMB) and compounds isolated from the stem bark of Morus mesozygia, namely 3 beta-acetoxyurs-12-en-11-one (1), moracin Q (2), moracin T (3), artocarpesin (4), cycloartocarpesin (5). moracin R (6), moracin U (8), moracin C (9), and moracin M (10). Materials and Methods: The liquid microdilution assay was used in the determination of the minimal inhibitory concentration (MIC) and the minimal microbicidal concentration (MMC), against nine bacterial and two fungal species. Results: The results of the MIC determination showed that the compounds 3,4,8 and 9 were able to prevent the growth of all tested microbial species. All other samples showed selective activities. Their inhibitory effects were noted on 90.9% studied organisms for the crude extract, 81.8% for compound 6, 72.7% for compound 10, 63.6% for compound 1, 54.5% for compound 5, and 45.5% for compound 2. The lowest MIC value of 39 mu g/ml was obtained with the crude extract against Escherichia coli. The corresponding value for compounds (5 mu g/ml) was registered with compound 9 on Shigella dysenteriae and compound 3 on E. coli, S. dysenteriae, Pseudomonas aeruginosa, Salmonella typhi and Bacillus cereus. The lowest MIC value (39 mu g/ml) observed with the crude extract (on E. coli) was only eightfold greater than that of gentamycin used as reference antibiotic (RA) while the corresponding value (5 mu g/ml) recorded with compounds 3 and 9 was equal to that of RA on the corresponding microorganisms. Conclusions: The obtained results highlighted the interesting antimicrobial potency of M. mesozygia as well as that of the studied compounds, and provided scientific basis for the traditional use of this species. (C) 2009 Elsevier Ireland Ltd. All rights reserved.