期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 126, 期 2, 页码 320-331出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2009.08.027
关键词
Anti-inflammatory; MAPK (Mitogen-activated protein kinase); MEK1/2 (Mitogen-activated protein kinase/ERK kinase); NF-kappa B (Nuclear Factor-kappaB); Scutellaria baicalensis
资金
- Korea Health Industry Development Institute [B050016]
- Korea Health Promotion Institute [B050016] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Council of Science & Technology (NST), Republic of Korea [K09050] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Aim of the Study: A herbal preparation using Scutellaria baicalensis (S. baicatensis) Georgi (Huang Qin, SB) was formulated to effectively protect cancer patients from inflammatory reactions. Although SB, is one of the most widely used herbs in oriental medicine for anti-inflammation, anti-cancer, anti-viral, antibacterial and tonifying the immune response, the underlying mechanism(s) by which these effects are induced remains unclear. Results: Here, we report that SB displays anti-inflammatory effects in a zymosan-induced mouse air-pouch model by reducing the expression of nitric oxide (NO), inducible NOS (iNOS), Cyclooxygenase2 (COX-2), Prostaglandin E2 (PGE2), Nuclear Factor-kappaB (NF-kappa B) and I kappa B alpha as well as inflammatory cytokines, such as IL-1 beta, IL-2, 1L-6, IL-12 and TNF-alpha. In a similar manner, SB also reduced the production of nitric oxide, PGE2, IL-1 beta, IL-2, IL-6, IL-12 and TNF-alpha, by decreasing the expression of iNOS, COX-2, I kappa B kinase alpha beta (IKK alpha beta) phosphorylation, I kappa B alpha and I kappa B alpha phosphorylation in LPS-treated Raw 264.7 cells. Additionally, SB interfered with the nuclear translocation of NF-kappa B p65 and p50, resulting in NF-kappa B-dependent transcriptional repression. We further demonstrate that SB attenuated the activity of c-Raf-1/MEK1/2, Erk1/2, p38 and JNK phosphorylation in LPS-treated Raw 264.7 cells. Conclusions: Taken together, these results confirm the strong anti-inflammatory properties of SB by inhibition of iNOS, COX-2, PGE2, IL-1 beta, 1L-2, IL-6, IL-12 and TNF-alpha expression. This was achieved through the down-regulation of IKK alpha beta, I kappa B alpha, NF-kappa B activation via suppression of c-Raf-1/MEK1/2 (Mitogen-activated protein kinase/ERK kinase) and MAP kinase phosphorylation in the zymosan-induced mice air-pouch and Raw 264.7 cells. These results support the use of SB herbs for its potent anti-inflammatory activity. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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