期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 119, 期 1, 页码 145-152出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2008.06.016
关键词
taheebo; Tabebuia avellanedae (Bignoniaceae); anti-inflammatory effects; cyclooxygenase II; arachidonic acid-induced ear edema
资金
- Nutraceutical Bio Brain Korea 21 Project of Kangwon National University
- KRF, Korea [2007-511-E00011]
- National Research Foundation of Korea [2007-511-E00011] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Aim of study: Tabebuia spp. (Bignoniaceae) are native to tropical rain forests throughout Central and South America and have long been used as a folk medicine to treat bacterial infection, blood coagulation. cancer and inflammatory diseases. In this study, we aimed to demonstrate the ethnopharmacological activity of Tabebuia avellanedae in various in vitro and in vivo inflammatory conditions. Materials and methods: To do this, LPS-stimulated macrophages and arachidonic acid or croton oil-induced mouse ear edema models were employed. Results: The water extract (taheebo) of Tabebuia avellanedae significantly suppressed the production of prostaglandin (PG) E-2 and nitric oxide (NO), and blocked the mRNA expression of their catalyzing enzymes (cyclooxygenase [COX)-II] and inducible NO synthase [iNOS], respectively), in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The blockade of inflammatory mediators by taheebo seemed to be the result of the interruption of extracellular signal-related kinase (ERK) activation, according to immunoblotting analysis and the NO assay, where LPS strongly induced the phosphorylation (a hallmark of activation) of ERK, and U0126, a selective ERK inhibitor, was found to strongly inhibit PGE(2) production. Similarly, oral administration of taheebo (100 mg/kg) for I week completely diminished mouse ear edema induced by arachidonic acid, an activator of COX-II, but not croton oil, an activator of lipoxygenase. Conclusions: These data suggest that the ethnopharmacological action of taheebo maybe due to its negative modulation of macrophage-mediated inflammatory responses by suppressing PGE(2) production. Thus, this water extract may be developed as a new therapeutic remedy for various inflammatory diseases such as arthritis and atherosclerosis. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据