期刊
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
卷 26, 期 5, 页码 728-733出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/14756366.2010.548811
关键词
Tyrosinase; suicide inactivation; tetrahydropterines
资金
- Ministerio de Educacion y Ciencia (Madrid, Spain) [BIO2009-12956]
- Fundacion Seneca (CARM, Murcia, Spain) [08856/PI/08, 08595/PI/08]
- Consejeria de Educacion (CARM, Murcia, Spain) [BIO-BMC 06/01-0004]
- Fundacion Caja Murcia (Murcia, Spain)
Tetrahydrobiopterin (BH4), methyl-tetrahydropterin (MBH4) and dimethyl-tetrahydropterin (DMBH 4) are oxidized by tyrosinase in a process during which the suicide inactivation of tyrosinase may occur. From the kinetic study of this process, lambda(SR)(Eox)((max)) (apparent maximum constant for the suicide inactivation), K-m(SR) (Michaelis constant for the substrate) and r (number of turnovers that the enzyme makes before the inactivation) can be obtained. From the results obtained, it can be deduced that the velocity of the inactivation governed by (lambda(SR)(Eox)((max))) and the potency of the same (lambda(SR)(Eox)((max))/K-m(SR)) follow the order: BH4 > MBH4 > DMBH4.
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