The effect of upper mesophilic temperature and feed-to-seed ratio on batch anaerobic digestion systems

This study investigated the effect of upper mesophilic temperature and feed-to-seed (F/S) ratio on anaerobic digestion using four 3.5 L batch-scale reactors. Initially, eight F/S ratios, ranging from 10/90 to 90/10, were explored at 37 degrees C, using a mixture of primary and secondary municipal sludge as feed. It was observed that the systems with low F/S ratios (40/60 and below) showed a stable performance while those with high ratios (50/50 and above) experienced the effect of organic overloading indicated by reduced removal of volatile solids (VS) in the feed, a drop in pH, volatile fatty acid (VFA) accumulation during the first 10 days of operation, and total gas production markedly lower than the corresponding theoretical values. Subsequently, the effect of temperature, in the 37 to 49 degrees C range, was studied at an F/S ratio of 20/80. Results revealed that an increase in temperature between 37 and 43 degrees C had a rather minimal effect on the process, with the exception of a moderate increase in total gas production. A further rise in the temperature in the 45 to 49 degrees C range however appeared to trigger an adverse effect evidenced by enhanced percent VSS reduction (possibly the result of cell lysis), VFA accumulation and an increase in the non-VFA total organic carbon (TOC) content. Therefore, it can be concluded that an operating temperature in the 37 to 43 degrees C range resulted in a stable and satisfactory reactor performance.