期刊
出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10934520802171675
关键词
silica nano-particles; Chlorella kessleri; size-dependent toxicity; cell structure
SiO2 nano-particles were found to exhibit size-dependent toxicity toward the alga, Chlorella kessleri. Small SiO2 nano-particles exhibit stronger toxicity: 50% inhibitory concentrations (IC50) value for 5 nm = 0.8 +/- 0.6%, 26 nm = 7.1 +/- 2.8%, and 78 nm = 9.1 +/- 4.7%. Enlargement of the cell body was observed by flow cytometry, which is due to the presence of structures that obstructed cell division. Optical and transmission microscopes were used to observe coagulated cells with incomplete division. Although the physiological effect of SiO2 nano-particles was not clear, SiO2 nano-particles are toxic, at least for algae in aquatic media. Under the transmission electron microscope, several amorphous structures appeared in the cells that were exposed to 5-nm silica nano-particles.
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