期刊
JOURNAL OF ENVIRONMENTAL ENGINEERING-ASCE
卷 138, 期 7, 页码 780-785出版社
ASCE-AMER SOC CIVIL ENGINEERS
DOI: 10.1061/(ASCE)EE.1943-7870.0000535
关键词
Disinfection; Microbes; Pathogens; Phage; Relative humidity; Spore; Virus
资金
- NASA Specialized Center for Research and Training (NSCORT)
Laboratory experiments were performed to demonstrate an experimental method for measuring the UV dose-response behavior among air-exposed microorganisms, and to quantify the effect of relative humidity on this behavior. These experiments included two nonpathogenic microorganisms as surrogates for airborne microbial pathogens: Bacillus subtilis spores and coliphage MS2. UV dose-response behavior of these microorganisms was measured as a function of relative humidity (RH). Microorganisms were transferred to membrane filters at less than monolayer coverage and were subjected to exposure to UV radiation delivered from a collimated beam to a planar surface in a humidity-controlled environment at two germicidally active wavelengths: 254 nm and 282 nm. Dose-response data for Bacillus subtilis spores and coliphage MS2 exposed to UV radiation were fit to the Phenotypic Persistence and External Shielding (PPES) model. B. subtilis spores showed a modest effect of RH on the UV dose-response behavior, whereas essentially no effect of RH was evident for air-exposed MS2 for their UV dose-response behavior at 254 nm or 282 nm. DOI: 10.1061/(ASCE)EE.1943-7870.0000535. (C) 2012 American Society of Civil Engineers.
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