期刊
JOURNAL OF ENDODONTICS
卷 40, 期 8, 页码 1194-1200出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.joen.2014.01.001
关键词
Biocompatibility; Biodentine; dental pulp cells; heme oxygenase-1; inflammatory response; nuclear factor E2-related factor-2; odontoblastic potential
资金
- National Research Foundation of Korea grant - Korean government (MSIP) [2012R1A5A2051384]
- Basic Science Research Program through the National Research Foundation of Korea - Ministry of Education, Science and Technology (MEST) [2011-0014231]
- Kyung Hee University [KHU-20131045]
- National Research Foundation of Korea [2011-0014231] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Introduction: The objective of this study was to evaluate the biocompatibility, inflammatory response, and odontoblastic potential of Biodentine (Septodont, Saint Maur des Fosses, France), Ortho-MTA (OMTA; BioMTA, Seoul, Korea), Angelus-MTA (AMTA; Angelus, Londrina, Brazil), and IRM (Dentsply Tulsa Dental, Tulsa, OK) in human dental pulp cells. The underlying signaling mechanisms were also investigated. Methods: Biocompatibilities were examined by the 3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyltetrazolium bromide assay. Differentiation was assessed by alkaline phosphatase activity, alizarin red S staining, and reverse-transcription polymerase chain reaction for marker genes. The levels of inflammatory mediators and cytokines were measured by reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Signal transduction analysis was performed by Western blotting. Results: Biodentine, OMTA, and AMTA showed favorable cell proliferation, alkaline phosphatase activity, formation of mineralized nodules, and expression of odontoblastic marker genes that were similar to those of IRM. The levels of proinflammatory mediators including nitric oxide, prostaglandin E-2 inducible nitric oxide synthase, and cyclooxygenase-2 were lower for Biodentine, OMTA, and AMTA compared with the IRM group. All test materials induced reactive oxygen species production and the expression of hemeoxygenase-1, nuclear factor E2-related factor-2, and mitogen-activated protein kinases. Conclusions: These data indicate for the first time that the biocompatibility, inflammatory response, and odontoblastic differentiation of Biodentine were similar to that of OMTA and AMTA in HDPCs, which suggests that Biodentine could be good alternative pulp capping agent.
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