期刊
JOURNAL OF ENDODONTICS
卷 34, 期 11, 页码 1360-1363出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.joen.2008.08.020
关键词
Calcium hydroxide; CGRP; cytokines; interleukin-1; neuropeptides; tumor necrosis factor
资金
- AAE Foundation
- [NIDCR K23 DE14864]
- [P01 DA16719]
- [R01 NS45186]
Calcium hydroxide, a widely used intracanal medicament, is known to exert an antimicrobial effect degrade bacterial-derived lipopolysaccharides. However, little is known about the effect of Ca(OH)(2) on endogenous inflammatory mediators such as interleukin-1 alpha (IL-1 alpha), tumor necrosis factor-alpha (TNF-alpha), and calcitonin gene-related peptide (CGRP). This is an important gap in knowledge because these inflammatory mediators play an important role in mediating the pathogenesis of periradicular periodontitis. We tested the hypothesis that Ca(OH)(2) denatures IL-1 alpha, TNF-alpha, and CGRP. Human IL-1 alpha (0.125 ng/mL), TNF-alpha (0.2 ng/mL), and CGRP (0.25 ng/mL) were incubated with Ca(OH)(2) (0.035 mg/mL) for 1-7 days. At the end of the incubation period, the pH of the samples was neutralized, and the concentrations of the mediators were measured by immunoassays. Data were analyzed with one-way analysis of variance and Bonferroni multiple comparison tests. The results indicate that Ca(OH)(2) denatures IL-1 alpha, TNF-alpha, and CGRP by 50%-100% during the testing periods (P<.001). We concluded that denaturation of these proinflammatory mediators is a potential mechanism by which Ca(OH), contributes to the resolution of periradicular periodontitis. (J Endod 2008;34:1360-1363)
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据