期刊
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION
卷 33, 期 2, 页码 96-102出版社
SPRINGER
DOI: 10.1007/BF03346561
关键词
3T3-L1 adipocytes; adiponectin; glucose turnover; insulin signaling; RNA interference
资金
- National Natural Science Foundation of China [30370671, 30771037]
- China Education Ministry [2003-19]
- Chongqing Municipal Education Commission [KJ 050304]
- National Institutes of Health [R01-DK 58895]
Adiponectin is a polypeptide hormone that is secreted by adipocytes with insulin-sensitizing and anti-inflammatory properties. The current study was to further investigate the role of adiponetin on glucose uptake and its underlying mechanism by down-regulation of adiponectin in 3T3-L1 adipocytes. Transfection of short hairpin RNA (shRNA)-vector significantly decreased adiponectin mRNA expression and its protein level in the cells. The down-regulation of adiponectin markedly reduced the cellular glucose uptake rate and increased intracellular triglyceride content. To study the mechanism of the physiologic action of adiponectin, several key regulatory factors in insulin signaling pathway were examined. The mRNA expression of insulin receptor substrate (IRS)-1 in both basal and insulin-stimulated states were down-regulated in the transfected cells (72% and 52% of controls, respectively), and the insulin-stimulated IRS-1 tyrosine phosphorylation was also significantly decreased. Adiponectin-deficient cells showed marked down-regulations of peroxisome proliferator-activated receptor alpha, glucose transporter (GLUT)-1, GLUT-4, hormone-sensitive lipase (HSL), and adipose triglyceride lipase. These results thus demonstrated that transfection of shRNA-vector effectively reduced the expression of adiponectin in 3T3-L1 adipocytes accompanied with a significant decrease in cellular glucose uptake rate and an increase in intracellular triglyceride content. Our data also suggested that adiponectin deficiency impair insulin action in vitro probably through the IRS-1 pathway, and increase intracellular fat accumulation partially through HSL down-regulation. (J. Endocrinol. Invest. 33: 96-102, 2010) (C) 2010, Editrice Kurtis
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据