期刊
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
卷 657, 期 1-2, 页码 84-90出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2011.03.026
关键词
Photosystem II; Herbicides; Picric acid; TNT; Screen-printed electrode
资金
- NSERC
- Defense RD Canada
- RCMP
- CBSA
- CATSA
The efficacy of a Photosystem II (PS II)-based biosensor for the detection of explosive compounds has been explored. The idea is based on the close similarities in the chemical structures of the widespread explosives and herbicides, with the latter known to inhibit functioning of the PS II by attaching to the binding site of the Q(B) mobile plastoquinone electron acceptor. The gold screen-printed electrodes (Au-SPE) functionalized with PS II-enriched particles were used for the detection of explosives in a droplet biosensor configuration. A crude preparation of PS II produced from spinach leaves, known as BBY particles, was employed to modify the Au-SPE working electrode employing BSA-glutaraldehyde-based immobilization procedure. Inhibition of the PS II functioning was detected by photo-electrochemical measurements in the presence of a mediator (either non-native quinone or ferricyanide). The biosensor was highly responsive to herbicides (as expected) as well as to picric acid, with limits of detection in the nanomolar range, but trace detection of trinitrotoluene (TNT) was not effective. The detection limit for picric acid was 25 nM as compared to similar to 400 nM for TNT with duroquinone mediator. Low affinity of PS II to TNT has been corroborated by means of DCPIP assay; possible reasons for low affinity are discussed. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据