期刊
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
卷 657, 期 1-2, 页码 28-33出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2011.03.004
关键词
Graphene; Pt; Hemoglobin; Direct electron transfer; Biosensor
资金
- National Natural Science Foundation of China [20905038, 20903057, 21073084, 20773065]
- National Basic Research Program of China [2009CB930600]
- Natural Science Foundation of Jiangsu province [08KJB150011, 09KJB150007]
- Opening Foundation of Key Laboratory of Analytical Chemistry for Life Science of Nanjing University [KLACLS08018]
A novel hemoglobin (Hb) electrode has been presented through adsorbing lib onto graphene-Pt nanocomposite. The graphene-Pt with uniform sheet-like structure was obtained by microwave-assisted method. Positions of Soret absorption band showed lib retained its natural configuration after adsorption onto graphene-Pt. The electrochemical behavior of the immobilized Hb was studied by cyclic voltammetry. The immobilized Hb showed a pair of stable and well-defined peaks at the formal potential of -0.26 V. As a model, a kind of novel third generation biosensor for H(2)O(2) was developed. The resultant heme-protein electrode exhibited electrocatalytical responses to the reduction of H(2)O(2), excellent stability, interference and good reproducibility. The presence of graphene enhanced the protein loadings, accelerates interfacial electron transfer and improves thermal stability of the adsorbed protein. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据