4.7 Article

pH-dependent electrochemical behavior of proteins with different isoelectric points on the nanostructured TiO2 surface

期刊

JOURNAL OF ELECTROANALYTICAL CHEMISTRY
卷 642, 期 2, 页码 109-114

出版社

ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2010.02.021

关键词

Electrochemical behavior; Proteins; Isoelectric points; Nanostructured TiO2

资金

  1. National Natural Science Foundation of China [20975075]
  2. Program for New Century Excellent Talents in University [NCET-06-0380]
  3. Scientific Research Foundation, State Education Ministry, China
  4. Nanometer Science Foundation of Shanghai [0952nm04900]

向作者/读者索取更多资源

Nanostructured materials are widely employed for facilitating electron transfer of proteins, and then constructing the third-generation biosensors. For further understanding the mechanism that nanomaterials facilitate electron transfer of proteins, nanostructured titanium dioxide (TiO2) film was prepared as an example for investigating the interaction between proteins and electrode surfaces. The TiO2 film was characterized by electrochemical methods, and the point of zero charge (pzc) was determined to be similar to 6.27. On the other hand, cytochrome c (cyt. c) and superoxide dismutase (SOD) were raised as model proteins because they have different isoelectric points of 10.5 and 4.9, respectively. Direct electron transfer of cyt. c and SOD was achieved at the nanostructured TiO2 surfaces at pH 7.0 and the proteins were verified to be stably immobilized onto the TiO2 films. Furthermore, electrochemical behavior of cyt. c and SOD modified on nanostructured TiO2 film was investigated in a series of different pH PBS solutions based on cyclic voltammetry. It was found that electron transfer of proteins strongly depended on pH of solutions, i.e., the surface charge of TiO2 film. This observation could be ascribed to the electrostatic interaction between proteins and surfaces. This is the first report to provide the insight in the relationship between electron transfer of proteins and interaction of surfaces charge. (C) 2010 Elsevier B.V. All rights reserved.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据