期刊
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
卷 634, 期 2, 页码 123-126出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2009.07.022
关键词
Carbon nanotubes; Chitosan; Glassy carbon electrode; DNA damage; Methylene blue sensor; Methylene blue electrochemistry
资金
- FONDECYT (Chile) [1080526]
- CONICET
- SECyT-UNC
- ANPCyT (Argentina)
In this work we report the optimization of a chitosan (CHI) and carbon nanotubes (CNT) modified electrode, CNT-CHI, to study the dsDNA interaction with methylene blue (MB), a well-known DNA intercalator. The analytical signal was the reduction current of the MB associated with the dsDNA confined at CNT-CHI modified glassy carbon electrode. The selected CNT-CHI film stabilizing treatment was immersion for 2 s in a 0.20% GTA solution. The effect of ionic strength was also analyzed, concluding that high concentration of NaCl (90 mM) benefits the intercalative process. Below these conditions, a linear response between intensity current and MB concentration was obtained. In conclusion. modifying the initial conditions of the assembling GCE/CHI-CNT/dsDNA electrode the intercalative process was favored opening a fast and cheap possibility to evaluate the toxicity of new synthesized molecules. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据