期刊
JOURNAL OF ECONOMIC ENTOMOLOGY
卷 106, 期 3, 页码 1098-1102出版社
ENTOMOLOGICAL SOC AMER
DOI: 10.1603/EC12308
关键词
Bacillus thuringiensis; receptor binding; ELISA; immunohistochemistry; Aedes albopictus
类别
资金
- National Natural Science Foundation of China [31071745, 31201574]
- Science Foundation of the Ministry of Education of China [20093515110010, 20093515120010]
- Transformation Fund for Agricultural Science and Technology Achievements [2010GB2C400212]
- National High Technology Research and Development Program 863 [2011AA10A203]
- Education Department Foundation of Fujian Province Distinguished Young Scholars in Universities of Fujian Province [JA12092]
- Key Project on the Science Fund for Distinguished Young Scholars of Fujian Agriculture and Forestry University [xjq201203]
- Service of Fujian Colleges and Universities for the Development of the West Strait [0b08b005]
- Institute of Entomology of the Academy of Sciences of the Czech Republic [Z50070508]
Bacillus thuringiensis (Bt) (Berliner) strain LLP29 produces a crystal protein Cyt1Aa6 toxic to mosquito vectors of human diseases. However, the susceptibility of Culex quinquefasciatus (Say) in the current study was 8.25 times higher than that of Aedes albopictus (Skuse) with this single protein Cyt1Aa6 purified from LLP29. To understand the mechanism of the novel mosquitocidal protein, the binding characteristic of brush border membrane vesicles from the two tested mosquitoes was investigated. Enzyme-linked immunosorbent assay showed that Cyt1Aa6 bound to the two mosquitoese brush border membrane vesicles. However, the titer of Ae. albopictus was a little higher than that of Cx. quinquefasciatus, with 3.21 and 2.91, respectively. Ligand Western blot analysis showed Cyt1Aa6 toxin specifically bound to the same three proteins (i.e., 68, 54, and 26 kDa) in the two mosquitoes, but one another protein, approximately to 37 kDa, could just be detected in Cx. quinquefasciatus. However, little difference was found in the test of immunohistochemistry. Cyt1Aa6 was detected in the midguts of both mosquitoes with histopathological changes. It would of great importance to the knowledge of the novel toxin against to Cx. quinquefasciatus and Ae. albopictus.
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