期刊
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
卷 24, 期 2, 页码 178-184出版社
ELSEVIER
DOI: 10.1016/S1773-2247(14)50029-9
关键词
Oral vaccine; Alginate hydrogel microspheres; Maltodextrin cryoprotectant; Protein; Bovine serum albumin; ESEM; In vitro release
资金
- Directorate General of Higher Education (DGHE)
- Indonesia for PhD studies at the University of Queensland
Oral administration of dry vaccine formulations is acknowledged to offer major clinical and logistical benefits by eliminating the cold chain required for liquid preparations. A model antigen, bovine serum albumin (BSA) was encapsulated in alginate microspheres using aerosolisation. Hydrated microspheres 25 to 65 mu m in size with protein loading of 3 3 % w/w were obtained. Environmental scanning electron microscopy indicated a stabilizing effect of encapsulated protein on alginate hydrogels revealed by an increase in dehydration resistance. Freeze drying of alginate microspheres without use of a cryoprotectant resulted in fragmentation and subsequent rapid loss of the majority of the protein load in simulated intestinal fluid in 2 h whereas intact microspheres were observed following freeze-drying of BSA-loaded microspheres in the presence of maltodextrin. BSA release from freeze-dried preparations was limited to less than 7 % in simulated gastric fluid over 2 h. while 90 % of the protein load was gradually released in simulated intestinal fluid over 10 h. SDS-PAGE analysis indicated that released BSA largely preserved its molecular weight. These findings demonstrate the potential for manufacturing freeze-dried oral vaccines using alginate microspheres.
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