期刊
NUCLEIC ACIDS RESEARCH
卷 43, 期 13, 页码 6270-6284出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkv574
关键词
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资金
- Ministry of Science and Technology of China [2015CB553906, 2013CB910501, 2013ZX09401-004-006]
- National Natural Science Foundation of China [81230051, 30830048, 31170711, 81321003]
- Beijing Natural Science Foundation [7120002]
- 111 Project of the Ministry of Education, Peking University [BMU20120314, BMU20130364]
- Leading Academic Discipline Project of Beijing Education Bureau
Polycomb repression complex 2 (PRC2) component EZH2 tri-methylates H3K27 and exerts epigenetic repression on target gene expression. EZH2-mediated epigenetic control of RNA polymerase II (Pol II) transcribed coding gene transcription has been well established. However, little is known about EZH2-mediated epigenetic regulation of RNA polymerase III (Pol III) transcription. Here we present a paradigm that EZH2 is involved in the repression of Pol III transcription via interaction with transcriptional factor complex IIIC (TFIIIC). EZH2 and H3K27me3 co-occupy the promoter of tRNA(Tyr), 5S rRNA and 7SL RNA genes. Depletion of EZH2 or inhibition of EZH2 methyltransferase activity led to upregulation of Pol III target gene transcription. EZH2-mediated repression of Pol III transcribed gene expression requires presence of SUZ12. SUZ12 was able to interact with TFIIIC complex and knockdown of SUZ12 decreased occupancy of EZH2 and H3K27me3 at the promoter of Pol III target genes. Our findings pointed out a previously unidentified role of PRC2 complex in suppressing transcription of Pol III transcribed non-translated RNA genes, putting Pol III on a new layer of epigenetic regulation.
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