期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 176, 期 7, 页码 2027-2038出版社
SPRINGER
DOI: 10.1007/s12010-015-1698-8
关键词
Snake venom; Phospholipase A(2); Antimicrobial; Naja naja; Human pathogenic bacteria
资金
- DST (department of Science and Technology), Govt of India [INT/SLP/P-007/2012]
- Balagangadharanatha Swamiji Institute for Technology (BGS-IT)
- Sri Adichunchangiri College of Pharmacy
Microbial resistance against antibiotics is considered as a potentially serious threat to public health. Therefore, there is much interest in developing new molecules with novel modes of action. In this study, when antimicrobial potential of an acidic protein-NN-XIa-PLA(2) (Naja naja venom phospholipase A(2) fraction-XIa) of N. naja venom was evaluated, it demonstrated potent bactericidal action against the human pathogenic strains. It inhibited more significantly, the gram-positive bacteria, when compared to gram-negative bacteria. The minimum inhibitory concentration (MIC) values ranged from 17 to 20 mu g/ml. It was interesting to observe that the NN-XIa-PLA(2) showed comparable antibacterial activity to the standard antibiotics used. It was found that there was a strong correlation between phospholipase A(2) (PLA(2)) activities, hemolytic, and antimicrobial activity. Further, it is found that in the presence of p-bromophenacyl bromide (p-BPB), there is a significant decrease in enzymatic activity and associated antimicrobial activities, suggesting that a strong correlation exists between catalytic activity and antimicrobial effects, which thereby destabilize the membrane bilayer. However, other mechanisms cannot be completely ruled out. Thus, these studies encourage further in-depth study on molecular mechanisms of antibacterial properties and thereby help in development of this protein into a possible therapeutic lead molecule for treating bacterial infections.
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