期刊
JOURNAL OF DERMATOLOGICAL SCIENCE
卷 61, 期 3, 页码 187-193出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jdermsci.2010.12.010
关键词
MicroRNA-221; Melanoma; Tumor marker; Serum
类别
资金
- Japan Society for the Promotion of Science [21890199]
- Government [21S-7(6)]
- Lydia O'Leary Memorial Foundation
- Grants-in-Aid for Scientific Research [21890199] Funding Source: KAKEN
Background: MicroRNA-221 (miR-221) is known to be abnormally expressed in malignant melanoma (MM) cells, and it favors the induction of the malignant phenotype through down-modulation of p27Kip1/CDKN1B and the c-KIT receptor. This suggests that the serum level of miR-221 might increase in patients with MM and thus could be used as a new tumor marker. Objective: To evaluate the possibility that the serum miR-221 level can be a marker of MM. Methods: Serum samples were obtained from 94 MM patients and 20 healthy controls. MicroRNAs were purified from serum, and miR-221 levels were measured by quantitative real-time polymerase chain reaction. Results: Circulating miR-221 was detectable and could be quantified in serum samples. MM patients had significantly higher miR-221 levels than healthy controls. Among the MM patients, the miR-221 levels were significantly increased in patients with stage I-IV MM compared to those with MM in situ, and the levels were correlated with tumor thickness. Moreover, a longitudinal study revealed a tendency for the miR-221 levels to decrease after surgical removal of the primary tumor, and to increase again at recurrence. Conclusions: Serum levels of miR-221 were significantly increased in MM patients and may be useful not only for the diagnosis of MM, but also for the differentiating MM in situ from stage I-IV MM, and for evaluating tumor progression and monitoring patients during the follow-up period. In addition, considering that the serum levels of miR-221 were correlated with tumor thickness, miR-221 might also be useful as a prognostic marker for patients with MM. (C) 2011 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.
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