期刊
ACTA BIOMATERIALIA
卷 15, 期 -, 页码 164-172出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2015.01.005
关键词
Collagen; VEGF; Tissue engineering; Angiogenesis; Microvasculature
资金
- NIAMS/NIH [R01AR060484, R21AR065124]
- DOD [W81XWH-12-1-0555]
- NSF IGERT fellowship
- HHMI Graduate Training Program (NB Med) fellowship
- NDSEG fellowship [23 CFR 168a]
In humans, high level of collagen remodeling is seen during normal physiological events such as bone renewal, as well as in pathological conditions, such as arthritis, tumor growth and other chronic wounds. Our lab recently discovered that collagen mimetic peptide (CMP) is able to hybridize with denatured collagens at these collagen remodeling sites with high affinity. Here, we show that the CMP's high binding affinity to denatured collagens can be utilized to deliver angiogenic signals to scaffolds composed of heat-denatured collagens (gelatins). We first demonstrate hybridization between denatured collagens and QKCMP, a CMP with pro-angiogenic QK domain. We show that high levels of QKCMP can be immobilized to a new artificial matrix containing both fibrous type I collagen and heat denatured collagen through triple helix hybridization, and that the QKCMP is able to stimulate early angiogenic response of endothelial cells (ECs). We also show that the QKCMP can bind to excised tissues from burn injuries in cutaneous mouse model, suggesting its potential for promoting neovascularization of burn wounds. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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