期刊
JOURNAL OF DENTAL RESEARCH
卷 93, 期 5, 页码 475-482出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/0022034514528211
关键词
cell proliferation; epithelial-mesenchymal interaction; morphogenesis; neural crest cells; odontogenesis; Kif3a
资金
- California Institute of Regenerative Medicine (CIRM) [TR1-01249]
- Pediatric Research Fund from Stanford's Lucile Packard Children's Hospital
- National Institutes of Health (NIH) [S10RR026645]
Many ciliopathies have clinical features that include tooth malformations but how these defects come about is not clear. Here we show that genetic deletion of the motor protein Kif3a in dental mesenchyme results in an arrest in odontogenesis. Incisors are completely missing, and molars are enlarged in Wnt1(Cre+)Kif3a(fl/fl) embryos. Although amelogenesis and dentinogenesis initiate in the molar tooth bud, both processes terminate prematurely. We demonstrate that loss of Kif3a in dental mesenchyme results in loss of Hedgehog signaling and gain of Wnt signaling in this same tissue. The defective dental mesenchyme then aberrantly signals to the dental epithelia, which prompts an up-regulation in the Hedgehog and Wnt responses in the epithelia and leads to multiple attempts at invagination and an expanded enamel organ. Thus, the primary cilium integrates Hedgehog and Wnt signaling between dental epithelia and mesenchyme, and this cilia-dependent integration is required for proper tooth development.
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